Match details

Record ID: NRN_CS540090
Database: nrnl1 Go to original record
Description: Sequence 8 from Patent WO2007012078. Sequence 6 from Patent WO2007020295. Sequence 7 from Patent WO2007011939. METHIONINE PRODUCING RECOMBINANT MICROORGANISMS. USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICRORGANISMS. Microorganisms with increased efficiency for methionine synthesis. Method of producing methionine in Corynebacteria by over-expressing enzymes of the pentose phosphate pathway. Coryneform bacteria with formate-THF-synthetase and/or glycine cleavage activity. Sequence 30 from Patent WO2008101850. Sequence 27 from Patent WO2008101857. Sequence 19 from Patent WO2008152016. Sequence 31 from patent US 8163532. Sequence 31 from Patent WO2009144270.
Taxon: unidentified
Original size: 6350 bp
Patents: WO2007012078 (lens, espacenet) WO2007020295 (lens, espacenet) WO2007011939 (lens, espacenet) WO2007011939 (lens, espacenet) JP2009501550 (lens, espacenet) JP2009501548 (lens, espacenet) JP2009504172 (lens, espacenet) JP2010518827 (lens, espacenet) JP2010518837 (lens, espacenet) WO2008101850 (lens, espacenet) WO2008101857 (lens, espacenet) WO2008152016 (lens, espacenet) US8163532 (lens, espacenet) WO2009144270 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2783 2955 + 173 bp

Amplicon: 173 bp
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc 

View other 57974 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.