Match details
| Record ID: |
NRN_CS007935 |
| Database: |
nrnl1 |
Go to original record |
| Description: |
Sequence 2 from Patent EP1502955. Sequence 2 from Patent WO2005014827. |
| Taxon: |
unidentified |
| Original size: |
11195 bp |
| Patents: |
EP1502955 (lens, espacenet)
WO2005014827 (lens, espacenet)
|
View other 10 matches for this record
| Method: |
QT-CON-00-005 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator. |
| Target: | pat-CaMV T-35S |
View other 222 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
3480 |
3627 |
- |
148 bp |
|
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgccgct
51 gaaatcacca gtctctctct acaaatctat ctctctctat aataatgtgt
101 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctca
View other 31 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
2.08e-09 |
| primerB |
100.0 |
24 bp |
0 |
0 |
148 |
125 |
5.36e-11 |
| probe |
95.5 |
22 bp |
0 |
1 |
36 |
56 |
1.03e-07 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
