Match details

Record ID: NRN_AX235900
Database: nrnl1 Go to original record
Description: Sequence 6 from Patent WO0164926. Sequence 3 from patent US 6395966. Sequence 6 from patent US 6743968. Method for preparing transformant of the coffee family plant, and transformed coffee plant. Methods and Compositions to Reduce or Eliminate Transmission of aTransgene. Methods and Compositions to Reduce or Eliminate Transmission of a Transgene. Sequence 65 from patent US 8022272.
Taxon: unidentified
Original size: 615 bp
Patents: WO0164926 (lens, espacenet) US6395966 (lens, espacenet) US6395966 (lens, espacenet) US6743968 (lens, espacenet) US2002144305 (lens, espacenet) JP2000245485 (lens, espacenet) JP2004512007 (lens, espacenet) US8022272 (lens, espacenet)

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Method: QL-ELE-00-022 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (bar) gene (Barbau-Piednoir et al., 2014).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 102 170 + 69 bp

Amplicon: 69 bp
1     cgtcaaccac tacatcgaga caagcacggt caacttccgt accgagccgc
51    aggaaccgca ggagtggac 

View other 2531 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 8.68e-11
primerB 100.0 19 bp 0 0 69 51 1.13e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.