Match details

Record ID: NRN_CQ887765
Database: nrnl1 Go to original record
Description: Sequence 46 from Patent WO2004063358. Sequence 46 from Patent WO2004063359. METHOD FOR THE GENETIC MODIFICATION OF ORGANISMS OF THE GENUS BLAKESLEA,CORRESPONDING ORGANISMS, AND THE USE OF THE SAME. METHOD FOR PRODUCING CAROTENOIDS OR THEIR PRECURSORS USINGGENETICALLY MODIFIED ORGANISMS OF THE BLAKESLEA GENUS,CAROTENOIDS OR THEIR PRECURSORS PRODUCED BY SAID METHOD ANDUSE THEREOF. Method for the genetic modification of organisms of the genus Blakeslea, corresponding organisms, and use of the same. Method for producing carotenoids or their precursors using genetically modified organisms of the Blakeslea genus, carotenoids or their precursors produced by said method and use thereof.
Taxon: unidentified
Original size: 21300 bp
Patents: WO2004063358 (lens, espacenet) WO2004063359 (lens, espacenet) JP2006513729 (lens, espacenet) JP2006515516 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 10269 10337 - 69 bp

Amplicon: 69 bp
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa 

View other 15381 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.