Match details

Record ID: NRN_CQ887765
Database: nrnl1 Go to original record
Description: Sequence 46 from Patent WO2004063358. Sequence 46 from Patent WO2004063359. METHOD FOR THE GENETIC MODIFICATION OF ORGANISMS OF THE GENUS BLAKESLEA,CORRESPONDING ORGANISMS, AND THE USE OF THE SAME. METHOD FOR PRODUCING CAROTENOIDS OR THEIR PRECURSORS USINGGENETICALLY MODIFIED ORGANISMS OF THE BLAKESLEA GENUS,CAROTENOIDS OR THEIR PRECURSORS PRODUCED BY SAID METHOD ANDUSE THEREOF. Method for the genetic modification of organisms of the genus Blakeslea, corresponding organisms, and use of the same. Method for producing carotenoids or their precursors using genetically modified organisms of the Blakeslea genus, carotenoids or their precursors produced by said method and use thereof.
Taxon: unidentified
Original size: 21300 bp
Patents: WO2004063358 (lens, espacenet) WO2004063359 (lens, espacenet) JP2006513729 (lens, espacenet) JP2006515516 (lens, espacenet)

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Method: QL-ELE-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 10269 10448 - 180 bp

Amplicon: 180 bp
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tagcgcgcaa actaggataa  

View other 29619 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.54e-09
primerB 100.0 20 bp 0 0 180 161 8.54e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.