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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS283703
Database: nrnl1 Go to original record
Description: Sequence 156 from Patent EP1645633. Sequence 147 from Patent EP1655364. Sequence 28 from Patent EP1662000. Sequence 100 from Patent EP1666599. Sequence 78 from Patent EP1669455. Sequence 60 from Patent EP1669456. Sequence 28 from patent US 7335813. Sequence 60 from patent US 7345216. Sequence 147 from patent US 7375209. Sequence 156 from patent US 7378572. Sequence 78 from patent US 7524948. Sequence 156 from patent US 8071749. Sequence 78 from Patent EP2072620. Sequence 156 from Patent EP2163632. Sequence 28 from Patent EP2163631. Sequence 78 from Patent EP2163634. Sequence 156 from Patent EP2166097. Sequence 156 from Patent EP2166098.
Taxon: unidentified
Original size: 8986 bp
Patents: EP1645633 (lens, espacenet) EP1655364 (lens, espacenet) EP1662000 (lens, espacenet) EP1666599 (lens, espacenet) EP1669455 (lens, espacenet) EP1669456 (lens, espacenet) US7335813 (lens, espacenet) US2006117408 (lens, espacenet) US7345216 (lens, espacenet) US2006130178 (lens, espacenet) US7375209 (lens, espacenet) US2006112452 (lens, espacenet) US7378572 (lens, espacenet) US2006080747 (lens, espacenet) US7524948 (lens, espacenet) US2006162025 (lens, espacenet) US8071749 (lens, espacenet) EP2072620 (lens, espacenet) EP2163632 (lens, espacenet) EP2163631 (lens, espacenet) EP2163634 (lens, espacenet) EP2166097 (lens, espacenet) EP2166098 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 8154 8368 + 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.