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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX467606
Database: nrnl1 Go to original record
Description: Sequence 16 from Patent WO0234926. Sequence 16 from Patent EP1867724. Sequence 16 from patent US 7049485. Sequence 16 from patent US 7371926. Sequence 16 from patent US 7569745. Sequence 16 from patent US 7423195. Sequence 16 from patent US 7696411. Sequence 16 from patent US 8093456.
Taxon: unidentified
Original size: 2521 bp
Patents: WO0234926 (lens, espacenet) EP1867724 (lens, espacenet) EP1867724 (lens, espacenet) US7371926 (lens, espacenet) US2006179513 (lens, espacenet) US7569745 (lens, espacenet) US2008235830 (lens, espacenet) US7423195 (lens, espacenet) US2006185037 (lens, espacenet) US7696411 (lens, espacenet) US8093456 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 1996 2064 + 69 bp
Amplicon: 69 bp 
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa

View other 15381 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.