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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX467606
Database: nrnl1 Go to original record
Description: Sequence 16 from Patent WO0234926. Sequence 16 from Patent EP1867724. Sequence 16 from patent US 7049485. Sequence 16 from patent US 7371926. Sequence 16 from patent US 7569745. Sequence 16 from patent US 7423195. Sequence 16 from patent US 7696411. Sequence 16 from patent US 8093456.
Taxon: unidentified
Original size: 2521 bp
Patents: WO0234926 (lens, espacenet) EP1867724 (lens, espacenet) EP1867724 (lens, espacenet) US7371926 (lens, espacenet) US2006179513 (lens, espacenet) US7569745 (lens, espacenet) US2008235830 (lens, espacenet) US7423195 (lens, espacenet) US2006185037 (lens, espacenet) US7696411 (lens, espacenet) US8093456 (lens, espacenet)

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Method: QL-ELE-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator.
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1957 2040 + 84 bp
Amplicon: 84 bp 
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.