Match details

Record ID: NRN_CQ924484
Database: nrnl1 Go to original record
Description: Sequence 11 from Patent EP1477557. Sequence 11 from patent US 6346655. Sequence 11 from patent US 6646184. Sequence 11 from patent US 7049421. Transgenic plant and methods. Transgenic plant resistant to mycotoxins and methods.
Taxon: unidentified
Original size: 12949 bp
Patents: EP1477557 (lens, espacenet) US6346655 (lens, espacenet) US6346655 (lens, espacenet) US6646184 (lens, espacenet) US2002162136 (lens, espacenet) JP2002540787 (lens, espacenet)

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 12798 12915 + 118 bp

Amplicon: 118 bp
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc 

View other 15178 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.22e-11
primerB 100.0 24 bp 0 0 118 95 4.22e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.