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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS566035
Database: nrnl1 Go to original record
Description: Sequence 22 from Patent WO2006039376. Sequence 22 from patent US 7323556. CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF. Sequence 22 from patent US 7695914. Sequence 22 from patent US 7696341. Sequence 22 from patent US 7875429. Sequence 22 from patent US 7875430. Sequence 22 from patent US 7888023. Sequence 22 from patent US 7888495. Sequence 22 from patent US 7897342. Sequence 22 from patent US 7932033. Sequence 22 from patent US 7956246.
Taxon: unidentified
Original size: 2987 bp
Patents: WO2006039376 (lens, espacenet) US7323556 (lens, espacenet) US2006070139 (lens, espacenet) JP2008514233 (lens, espacenet) US7695914 (lens, espacenet) US7696341 (lens, espacenet) US7875429 (lens, espacenet) US7875430 (lens, espacenet) US7888023 (lens, espacenet) US7888495 (lens, espacenet) US7897342 (lens, espacenet) US7932033 (lens, espacenet) US7956246 (lens, espacenet)

View other 4 matches for this record

Method: QL-ELE-00-021 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (pat) gene (Barbau-Piednoir et al., 2014).
Target:pat

View other 1706 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 52 160 + 109 bp
Amplicon: 109 bp 
1     ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac
51    tttaggacag agccacaaac accacaagag tggattgatg atctagagag
101   gttgcaaga

View other 1557 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 5.47e-09
primerB 100.0 25 bp 0 0 109 85 1.21e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.