Match details

Record ID: NRN_CS354155
Database: nrnl1 Go to original record
Description: Sequence 17 from Patent WO2006024509. Sequence 1 from Patent WO2006136596. Disarmed Agrobacterium strains, Ri-plasmids, and methods of transformation based thereon. Sequence 17 from patent US 7989678. Sequence 1 from patent US 7994399. Sequence 1 from Patent EP2159289.
Taxon: unidentified
Original size: 15997 bp
Patents: WO2006024509 (lens, espacenet) WO2006136596 (lens, espacenet) JP2008511294 (lens, espacenet) US7989678 (lens, espacenet) US7994399 (lens, espacenet) EP2159289 (lens, espacenet)

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4494 4611 + 118 bp

Amplicon: 118 bp
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.22e-11
primerB 100.0 24 bp 0 0 118 95 4.22e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.