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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX481630
Database: nrnl1 Go to original record
Description: Sequence 20 from Patent WO02057465. Sequence 20 from Patent WO02057464. Sequence 38 from Patent WO03093482. Sequence 27 from Patent WO2004076617. Sequence 64 from Patent WO2004087902. Method for producing polyunsaturated fatty acids, novel biosynthesis genes and novel plant expression constructs. Sequence 27 from patent US 7537920. Sequence 20 from patent US 7615679. Sequence 38 from patent US 7893320. Sequence 20 from patent US 8088974.
Taxon: unidentified
Original size: 12093 bp
Patents: WO02057465 (lens, espacenet) WO02057464 (lens, espacenet) WO03093482 (lens, espacenet) WO2004076617 (lens, espacenet) WO2004087902 (lens, espacenet) JP2004530418 (lens, espacenet) US7537920 (lens, espacenet) US2006168687 (lens, espacenet) US7615679 (lens, espacenet) US2004053379 (lens, espacenet) US7893320 (lens, espacenet) US8088974 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 9642 9856 - 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.