Match details

Record ID: NRN_EA501372
Database: nrnl1 Go to original record
Description: Sequence 60 from patent US 7361813. Corn Event MIR604. Corn event MIR604. Sequence 60 from patent US 7897748. Sequence 46 from Patent EP2080805. Sequence 6 from Patent WO2009112508. Sequence 41 from Patent WO2009150170. Sequence 44 from Patent WO2010036946. Sequence 60 from Patent EP2281447. JP 2011516036-A/6: A method for the production of human recombinant lysosomal enzymes in a cereal endosperm. Sequence 60 from Patent EP2289311. Sequence 45 from Patent WO2011158242.
Taxon: unidentified
Original size: 253 bp
Patents: US7361813 (lens, espacenet) US2005216970 (lens, espacenet) JP2007530036 (lens, espacenet) US7897748 (lens, espacenet) EP2080805 (lens, espacenet) WO2009112508 (lens, espacenet) WO2009150170 (lens, espacenet) WO2010036946 (lens, espacenet) EP2281447 (lens, espacenet) JP2011516036 (lens, espacenet) EP2289311 (lens, espacenet) WO2011158242 (lens, espacenet)

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Method: QL-ELE-00-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 111 194 + 84 bp

Amplicon: 84 bp
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa 

View other 30799 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.