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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ990402
Database: nrnl1 Go to original record
Description: Sequence 80 from Patent WO2004113528. Sequence 80 from Patent WO2006060699. Sequence 80 from patent US 7358418. ISOFORMS OF EIF-5A:SENESCENCE-INDUCED EIF5A. WOUNDING-INDUCED EIF-5A;GROWTH EIF-5A;AND DHS. ISOFORMS OF EIF-5A: SENESCENCE-INDUCED EIF5A;WOUNDING-INDUCED EIF-5A; GROWTH EIF-5A; AND DHS. METHODS OF INCREASING SEED YIELD. Sequence 80 from patent US 7663028. Sequence 80 from patent US 8232455.
Taxon: unidentified
Original size: 319 bp
Patents: WO2004113528 (lens, espacenet) WO2006060699 (lens, espacenet) US7358418 (lens, espacenet) US2005155110 (lens, espacenet) JP2007524384 (lens, espacenet) JP2008521445 (lens, espacenet) US7663028 (lens, espacenet) US8232455 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 13 135 + 123 bp
Amplicon: 123 bp 
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga

View other 3472 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.84e-09
primerB 100.0 25 bp 0 0 123 99 1.30e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.