Match details

Record ID: NRN_BD284966
Database: nrnl1 Go to original record
Description: Method for detecting interaction between substance and protein, method for screening protein interacting with substance, and method for forming complex of substance and protein interacting with the substance. Method for detecting interaction between substance and protein, method for screening protein interacting with substance, and method for forming complex of substance and protein interacting with the substance. C-terminal modified protein and process for producing the same, modifying agent and translation template to be used in produfing C-terminal modified protein, and method of detecting protein interaction with the use of C-terminal modified protein. Translation template and library thereof, protein synthesized therefrom and protein library, and element constituting them, and method for producing and using them. Protein forming complex with c-Fos protein, and, nucleic acid encoding the same, and method for using them. Method for producing and method for using library of homologizing molecule and constitutional elements thereof. Sequence 168 from patent US 7670787.
Taxon: unidentified
Original size: 5172 bp
Patents: WO03014734 (lens, espacenet) WO03014734 (lens, espacenet) WO03048363 (lens, espacenet) WO03106675 (lens, espacenet) WO2004053121 (lens, espacenet) WO2004053127 (lens, espacenet) US7670787 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3387 3601 + 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagaa catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

View other 163 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.