JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CQ945863
Database:	nrnl1	Go to original record
Description:	Sequence 25 from Patent WO2004099447. Sequence 25 from patent US 7288643. Corn Event TC1507 and Methods for Detection Thereof. Sequence 25 from patent US 7417132. Sequence 25 from patent US 7435807. Sequence 25 from patent US 7449564. Sequence 25 from patent US 7514544. Sequence 25 from patent US 7989607.
Taxon:	unidentified
Original size:	6186 bp
Patents:	WO2004099447 (lens, espacenet) US7288643 (lens, espacenet) US2005039226 (lens, espacenet) JP2006525028 (lens, espacenet) US7417132 (lens, espacenet) US2008015346 (lens, espacenet) US7435807 (lens, espacenet) US7435807 (lens, espacenet) US7449564 (lens, espacenet) US2008132690 (lens, espacenet) US7514544 (lens, espacenet) US2008051569 (lens, espacenet) US7989607 (lens, espacenet)

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Method:	QL-CON-00-005	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target:	pat-CaMV T-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5731	5939	+	209 bp

Amplicon:

209 bp

1     atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51    gctcctccaa ggccagttag gccagttacc cagatctgag tcgacctgca
101   ggcatgccgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
151   taataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
201   tttcgctca

View other 31 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	2.97e-09
primerB	100.0	23 bp	0	0	209	187	2.60e-10

Match details