Match details

Record ID: NRN_AX930606
Database: nrnl1 Go to original record
Description: Sequence 78 from Patent WO03087386. Sequence 66 from Patent WO2004024932. Sequence 74 from Patent WO2004024933. Sequence 74 from Patent WO2004024931. Sequence 12 from Patent WO2005059144. Sequence 10 from Patent WO2005059154. Sequence 10 from Patent WO2005059139. Sequence 29 from Patent WO2005059093. Sequence 8 from Patent WO2005116228. Sequence 10 from Patent WO2005121349. Sequence 68 from Patent WO2006069711. Sequence 68 from Patent EP1908841. Sequence 29 from Patent EP1908842. Sequence 12 from Patent EP1918378. Sequence 66 from patent US 7238502. Sequence 74 from patent US 7282357. Sequence 74 from patent US 7381549. P EF-TU Expression units. PSOD EXPRESSION UNITS. METHODS FOR THE PREPARATION OF A FINE CHEMICAL BY FERMENTATION. METHODS FOR THE PREPARATION OF LYSINE BY FERMENTATION OF CORYNEBACTERIUM GLUTAMICUM. METHODS FOR THE PREPARATION OF A FINE CHEMICAL BY FERMENTATION. MetH. Process for the production of sulfur-containing fine chemicals. MetF. MetA. METHODS FOR THE PREPARATION OF AFINE CHEMICAL BY FERMENTATION. P EF-TU-Expressionseinheiten. Psod-Expressionseinheiten. Fermentative production of fine chemicals. METHODS FOR THE PREPARATION OF A. METHODS FOR THE PREPARATION OF A. Methods for producing sulphurous fine chemicals. Method for the production by fermentation of sulphur-containing fine chemicals(METF). Method for zymotic production of fine chemicals containing sulphur (META). Method for zymotic production of fine chemicals containing sulphur (METY). Fermentative production of fine chemicals. MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION. PSOD EXPRESSION UNITS. P EF-TU Expression units. METHODS FOR THE PREPARATION OF A FINE CHEMICAL BY FERMENTATION. Sequence 10 from Patent EP1939296. Sequence 78 from patent US 7485444. Sequence 29 from patent US 7785779. Sequence 68 from patent US 7968699. Sequence 29 from patent US 8044191. Sequence 10 from patent US 8048651. Sequence 12 from patent US 8071365. JP 2008212157-A/68: MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION.
Taxon: unidentified
Original size: 5860 bp
Patents: WO03087386 (lens, espacenet) WO2004024932 (lens, espacenet) WO2004024933 (lens, espacenet) WO2004024931 (lens, espacenet) WO2005059144 (lens, espacenet) WO2005059154 (lens, espacenet) WO2005059139 (lens, espacenet) WO2005059093 (lens, espacenet) WO2005116228 (lens, espacenet) WO2005121349 (lens, espacenet) WO2006069711 (lens, espacenet) EP1908841 (lens, espacenet) EP1908842 (lens, espacenet) EP1918378 (lens, espacenet) US7238502 (lens, espacenet) US2006003425 (lens, espacenet) US7282357 (lens, espacenet) US2006068476 (lens, espacenet) US7381549 (lens, espacenet) US2005260721 (lens, espacenet) JP2007514425 (lens, espacenet) JP2007514426 (lens, espacenet) JP2007514436 (lens, espacenet) JP2007514437 (lens, espacenet) JP2007514439 (lens, espacenet) JP2005522218 (lens, espacenet) JP2008500823 (lens, espacenet) JP2008523834 (lens, espacenet) JP2008212154 (lens, espacenet) JP2008212155 (lens, espacenet) JP2008259505 (lens, espacenet) EP1939296 (lens, espacenet) US7485444 (lens, espacenet) US2006223149 (lens, espacenet) US7785779 (lens, espacenet) US7968699 (lens, espacenet) US8044191 (lens, espacenet) US8048651 (lens, espacenet) US8071365 (lens, espacenet) JP2008212157 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2287 2501 + 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.