Match details

Record ID: NRN_CS566034
Database: nrnl1 Go to original record
Description: Sequence 21 from Patent WO2006039376. Sequence 21 from patent US 7323556. CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF. Sequence 21 from patent US 7695914. Sequence 21 from patent US 7696341. Sequence 21 from patent US 7875429. Sequence 21 from patent US 7875430. Sequence 21 from patent US 7888023. Sequence 21 from patent US 7888495. Sequence 21 from patent US 7897342. Sequence 21 from patent US 7932033. Sequence 21 from patent US 7956246.
Taxon: unidentified
Original size: 3594 bp
Patents: WO2006039376 (lens, espacenet) US7323556 (lens, espacenet) US2006070139 (lens, espacenet) JP2008514233 (lens, espacenet) US7695914 (lens, espacenet) US7696341 (lens, espacenet) US7875429 (lens, espacenet) US7875430 (lens, espacenet) US7888023 (lens, espacenet) US7888495 (lens, espacenet) US7897342 (lens, espacenet) US7932033 (lens, espacenet) US7956246 (lens, espacenet)

Method: QT-EVE-ZM-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event 59122 (Mazzara et al., 2006).
Target:DAS-59122-7

View other 71 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2573 2658 + 86 bp

Amplicon: 86 bp
1     gggataagca agtaaaagcg ctcaaacact gatagtttaa actgaaggcg
51    ggaaacgaca atctgatcat gagcggagaa ttaagg 

View other 71 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.10e-10
primerB 100.0 24 bp 0 0 86 63 3.22e-11
probe 100.0 26 bp 0 0 36 61 2.76e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.