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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS158625
Database: nrnl1 Go to original record
Description: Sequence 13 from Patent WO2005080544. CELL/TISSUE CULTURING DEVICE, SYSTEM AND METHOD. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. CELL/TISSUE CULTURING DEVICE, SYSTEM AND METHOD. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE. Sequence 13 from patent US 7951557. Sequence 13 from Patent EP2220105. JP 2011184445-A/10: PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE.
Taxon: unidentified
Original size: 2186 bp
Patents: WO2005080544 (lens, espacenet) JP2007522824 (lens, espacenet) JP2006524506 (lens, espacenet) JP2010525806 (lens, espacenet) US7951557 (lens, espacenet) EP2220105 (lens, espacenet) JP2011184445 (lens, espacenet)

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 92 286 + 195 bp
Amplicon: 195 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 317 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.10e-08
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.