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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS646846
Database: nrnl1 Go to original record
Description: Sequence 101 from Patent WO2007061845. Sequence 32 from Patent WO2008147935. Sequence 101 from patent US 8049062. Sequence 111 from patent US 8084074. Sequence 32 from patent US 8143473. Sequence 32 from patent US 8143476. Sequence 32 from patent US 8153859. Sequence 32 from Patent WO2009143401. Sequence 32 from Patent WO2009143398. Sequence 27 from Patent WO2010114989. Sequence 8 from Patent WO2011008510.
Taxon: unidentified
Original size: 13268 bp
Patents: WO2007061845 (lens, espacenet) WO2008147935 (lens, espacenet) US8049062 (lens, espacenet) US8084074 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet) WO2010114989 (lens, espacenet) WO2011008510 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1425 1639 - 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.