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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS646846
Database: nrnl1 Go to original record
Description: Sequence 101 from Patent WO2007061845. Sequence 32 from Patent WO2008147935. Sequence 101 from patent US 8049062. Sequence 111 from patent US 8084074. Sequence 32 from patent US 8143473. Sequence 32 from patent US 8143476. Sequence 32 from patent US 8153859. Sequence 32 from Patent WO2009143401. Sequence 32 from Patent WO2009143398. Sequence 27 from Patent WO2010114989. Sequence 8 from Patent WO2011008510.
Taxon: unidentified
Original size: 13268 bp
Patents: WO2007061845 (lens, espacenet) WO2008147935 (lens, espacenet) US8049062 (lens, espacenet) US8084074 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet) WO2010114989 (lens, espacenet) WO2011008510 (lens, espacenet)

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2048 2242 - 195 bp
Amplicon: 195 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 8169 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.10e-08
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.