Match details

Record ID: NRN_AX110663
Database: nrnl1 Go to original record
Description: Sequence 1396 from Patent WO0123604. Sequence 1 from Patent WO2006034061. Sequence 13 from Patent WO2006106193. Sequence 24 from patent US 6878531. Sequence 1 from patent US 6977178. Sequence 1 from patent US 7011943. Sequence 1 from patent US 7282361. System for Automated Transgenic Screening. System for Automated Transgenic Screening. LINEAR DNA FRAGMENT FOR DEVELOPING A NOVEL STRAIN REMOVED ASPECIFIC REGION OF CHROMOSOME AND METHOD FOR PREPARING A NOVELSTRAIN REMOVED A SPECIFIC REGION OF CHROMOSOME USING THE SAME. Neomycin resistance gene and method of selecting transgenic strain using the same. Synthetic nucleic acid molecule and methods of preparation. An Organ Generation Method Using Blastocyst Complementation. Expression vector for multiple gene expression. SOMATIC HYPERMUTATION SYSTEMS. Sequence 1 from Patent EP1978110. Sequence 1 from patent US 7728118. Sequence 1 from patent US 8008006. Sequence 1396 from patent US 8114601. Sequence 1396 from patent US 8182996. Sequence 7 from Patent WO2009080720. Sequence 4 from Patent WO2009137136. Sequence 7 from Patent EP2180058. JP 2003511015-A/1396: HIGHLY CONSERVED GENES AND THEIR USE TO GENERATE SPECIES-SPECIFIC, GENUS-SPECIFIC, FAMILY-SPECIFIC, GROUP-SPECIFIC AND UNIVERSAL NUCLEIC ACID PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY ALGAL, ARCHAEAL, BACTERIAL, FUNGAL AND PARASITICAL MICROOR-GANISMS FROM CLINICAL SPECIMENS FOR DIAGNOSIS. JP 2011505859-A/7: Organic compounds. JP 2012506252-A/7: Meganuclease recombination system. WO 2012081628-A/19: Protein production method. WO 2012081629-A/6: Protein production method using weakend drug resistant gene. Sequence 3 from Patent WO2011101696. Sequence 3 from Patent WO2011101811.
Taxon: unidentified
Original size: 795 bp
Patents: WO0123604 (lens, espacenet) WO2006034061 (lens, espacenet) WO2006106193 (lens, espacenet) US6878531 (lens, espacenet) US6878531 (lens, espacenet) US6977178 (lens, espacenet) US2003165922 (lens, espacenet) US7011943 (lens, espacenet) US2002177137 (lens, espacenet) US7282361 (lens, espacenet) US2005170423 (lens, espacenet) JP2004508057 (lens, espacenet) JP2005245289 (lens, espacenet) JP2008513021 (lens, espacenet) WO2008102602 (lens, espacenet) JP2010088332 (lens, espacenet) JP2010520748 (lens, espacenet) EP1978110 (lens, espacenet) US7728118 (lens, espacenet) US8008006 (lens, espacenet) US8114601 (lens, espacenet) US8182996 (lens, espacenet) WO2009080720 (lens, espacenet) WO2009137136 (lens, espacenet) EP2180058 (lens, espacenet) JP2003511015 (lens, espacenet) JP2011505859 (lens, espacenet) JP2012506252 (lens, espacenet) WO2012081628 (lens, espacenet) WO2012081629 (lens, espacenet) WO2011101696 (lens, espacenet) WO2011101811 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 300 472 + 173 bp

Amplicon: 173 bp
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc 

View other 57974 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.