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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ945862
Database: nrnl1 Go to original record
Description: Sequence 24 from Patent WO2004099447. Sequence 24 from patent US 7288643. Corn Event TC1507 and Methods for Detection Thereof. Sequence 24 from patent US 7417132. Sequence 24 from patent US 7435807. Sequence 24 from patent US 7449564. Sequence 24 from patent US 7514544. Sequence 24 from patent US 7989607. Sequence 3 from Patent EP2513326.
Taxon: unidentified
Original size: 11361 bp
Patents: WO2004099447 (lens, espacenet) US7288643 (lens, espacenet) US2005039226 (lens, espacenet) JP2006525028 (lens, espacenet) US7417132 (lens, espacenet) US2008015346 (lens, espacenet) US7435807 (lens, espacenet) US7435807 (lens, espacenet) US7449564 (lens, espacenet) US2008132690 (lens, espacenet) US7514544 (lens, espacenet) US2008051569 (lens, espacenet) US7989607 (lens, espacenet) EP2513326 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 7952 8074 + 123 bp
Amplicon: 123 bp 
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga

View other 3472 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.84e-09
primerB 100.0 25 bp 0 0 123 99 1.30e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.