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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS249133
Database: nrnl1 Go to original record
Description: Sequence 5 from Patent WO2005121347. Sequence 34 from Patent WO2006009659. Sequence 24 from Patent WO2007127381. Sequence 34 from patent US 7294756. Sequence 104 from Patent WO2008147935. Sequence 34 from patent US 7476778. Sequence 24 from patent US 7943823. Sequence 24 from patent US 8058517. Sequence 104 from patent US 8143473. Sequence 195 from patent US 8143476. Sequence 248 from patent US 8153859. Sequence 323 from patent US 8153859. Sequence 79 from patent US 8168858. Sequence 248 from Patent WO2009143401. Sequence 323 from Patent WO2009143401. Sequence 195 from Patent WO2009143398. Sequence 4 from Patent WO2011008510. Sequence 24 from Patent EP2405012.
Taxon: unidentified
Original size: 5303 bp
Patents: WO2005121347 (lens, espacenet) WO2006009659 (lens, espacenet) WO2007127381 (lens, espacenet) WO2007127381 (lens, espacenet) US7294756 (lens, espacenet) US2006005280 (lens, espacenet) WO2008147935 (lens, espacenet) US7476778 (lens, espacenet) US2008172758 (lens, espacenet) US7943823 (lens, espacenet) US8058517 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) US8153859 (lens, espacenet) US8168858 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet) WO2011008510 (lens, espacenet) EP2405012 (lens, espacenet)

View other 11 matches for this record

Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2488 2569 + 82 bp
Amplicon: 82 bp 
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

View other 26489 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.