Match details

Record ID: NRN_CS646791
Database: nrnl1 Go to original record
Description: Sequence 46 from Patent WO2007061845. Sequence 82 from Patent WO2007061742. Sequence 94 from Patent WO2007136876. Sequence 93 from Patent WO2007136877. DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 110 from Patent WO2008124048. Sequence 82 from patent US 7645604. Sequence 82 from patent US 8048653. Sequence 46 from patent US 8049062. Sequence 94 from patent US 8049070.
Taxon: unidentified
Original size: 8671 bp
Patents: WO2007061845 (lens, espacenet) WO2007061742 (lens, espacenet) WO2007136876 (lens, espacenet) WO2007136876 (lens, espacenet) WO2007136877 (lens, espacenet) WO2007136877 (lens, espacenet) JP2009517019 (lens, espacenet) JP2010523113 (lens, espacenet) WO2008124048 (lens, espacenet) US7645604 (lens, espacenet) US8048653 (lens, espacenet) US8049062 (lens, espacenet) US8049070 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2471 2552 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.