Match details

Record ID: NRN_CS566040
Database: nrnl1 Go to original record
Description: Sequence 27 from Patent WO2006039376. Sequence 27 from patent US 7323556. CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF. Sequence 27 from patent US 7695914. Sequence 27 from patent US 7696341. Sequence 27 from patent US 7875429. Sequence 27 from patent US 7875430. Sequence 27 from patent US 7888023. Sequence 27 from patent US 7888495. Sequence 27 from patent US 7897342. Sequence 27 from patent US 7932033. Sequence 27 from patent US 7956246.
Taxon: unidentified
Original size: 2830 bp
Patents: WO2006039376 (lens, espacenet) US7323556 (lens, espacenet) US2006070139 (lens, espacenet) JP2008514233 (lens, espacenet) US7695914 (lens, espacenet) US7696341 (lens, espacenet) US7875429 (lens, espacenet) US7875430 (lens, espacenet) US7888023 (lens, espacenet) US7888495 (lens, espacenet) US7897342 (lens, espacenet) US7932033 (lens, espacenet) US7956246 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1699 1780 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.