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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_A84753
Database: nrnl1 Go to original record
Description: Sequence 5 from Patent WO9844138. Sequence 5 from patent US 6140554. Improvements relating to the specificity of gene expression,. JP 2010029196-A/5: Improvements Relating to the Specificity of Gene Expression.
Taxon: unidentified
Original size: 2319 bp
Patents: WO9844138 (lens, espacenet) US6140554 (lens, espacenet) JP2001517076 (lens, espacenet) JP2010029196 (lens, espacenet)

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Method: QL-ELE-00-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (L 00.00-31, 1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 1 2069 2247 + 179 bp
Amplicon: 179 bp 
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaatt atgcgcgcaa actaggata

View other 23 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.49e-09
primerB 100.0 17 bp 0 0 179 163 3.95e-07

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.