Match details
Record ID:
NRN_CQ924483
Database:
nrnl1
Go to original record
Description:
Sequence 10 from Patent EP1477557. Sequence 10 from patent US 6346655. Sequence 10 from patent US 6646184. Sequence 10 from patent US 7049421. Transgenic plant and methods. Transgenic plant resistant to mycotoxins and methods.
Taxon:
unidentified
Original size:
13737 bp
Patents:
EP1477557 (lens , espacenet )
US6346655 (lens , espacenet )
US6346655 (lens , espacenet )
US6646184 (lens , espacenet )
US2002162136 (lens , espacenet )
JP2002540787 (lens , espacenet )
View other 18 matches for this record
Method:
QT-CON-00-005
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target: pat-CaMV T-35S
View other 222 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
10725
10873
-
149 bp
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgcccgc
51 tgaaatcacc agtctctctc tacaaatcta tctctctcta taataatgtg
101 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctca
View other 150 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.09e-09
primerB
100.0
24 bp
0
0
149
126
5.39e-11
probe
100.0
22 bp
0
0
36
57
6.20e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.