Match details

Record ID: NRN_CQ924483
Database: nrnl1 Go to original record
Description: Sequence 10 from Patent EP1477557. Sequence 10 from patent US 6346655. Sequence 10 from patent US 6646184. Sequence 10 from patent US 7049421. Transgenic plant and methods. Transgenic plant resistant to mycotoxins and methods.
Taxon: unidentified
Original size: 13737 bp
Patents: EP1477557 (lens, espacenet) US6346655 (lens, espacenet) US6346655 (lens, espacenet) US6646184 (lens, espacenet) US2002162136 (lens, espacenet) JP2002540787 (lens, espacenet)

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Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 11466 11540 - 75 bp

Amplicon: 75 bp
1     aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
51    aatcccacta tccttcgcaa gaccc 

View other 5753 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 9.50e-11
primerB 100.0 20 bp 0 0 75 56 3.68e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.