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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS353195
Database: nrnl1 Go to original record
Description: Sequence 44 from Patent WO2006069201. Sequence 233 from patent US 7598429. Sequence 59 from patent US 7868229. Sequence 80 from patent US 7888558. Sequence 26 from patent US 7939715. Sequence 1586 from patent US 7956242. Sequence 4837 from patent US 7960612. Sequence 61 from patent US 8022274. Sequence 5100 from patent US 8030546.
Taxon: unidentified
Original size: 4276 bp
Patents: WO2006069201 (lens, espacenet) US7598429 (lens, espacenet) US2006195944 (lens, espacenet) US7868229 (lens, espacenet) US7888558 (lens, espacenet) US7939715 (lens, espacenet) US7956242 (lens, espacenet) US7960612 (lens, espacenet) US8022274 (lens, espacenet) US8030546 (lens, espacenet)

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Method: QL-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:CaMV P-35S

View other 10536 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 726 855 + 130 bp
Amplicon: 130 bp 
1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcggcg gccgcaagac ccttcctcta
101   tataaggaag ttcatttcat ttggagagga

View other 16 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.95e-09
primerB 100.0 25 bp 0 0 130 106 1.37e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.