Match details
| Record ID: |
NRN_BD295580 |
| Database: |
nrnl1 |
Go to original record |
| Description: |
Nucleic acid fragment for competitive PCR, and kit and method for quantitative determination of recombinant gene. |
| Taxon: |
unidentified |
| Original size: |
1135 bp |
| Patents: |
WO03027283 (lens, espacenet)
|
View other 10 matches for this record
| Method: |
QT-TAX-ZM-006 |
Go to GMOMETHODS record |
| Description: | Quantitative PCR method for detection of maize starch synthase IIb gene |
| Target: | maize starch synthase IIb |
View other 41 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
1 |
172 |
+ |
172 bp |
|
1 ctcccaatcc tttgacatct gctccgaagc aaagtcagag cgctgcaatg
51 caaaacggaa ttagcactca gctgtgaatg tcgagtgggg gcagcagcgc
101 gagcaccgcc gcgccggtgt ccggacccaa agctgatcat ccatcagctc
151 ctgtcaccaa gagagaaatc ga
View other 1 match with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
7.20e-10 |
| primerB |
100.0 |
23 bp |
0 |
0 |
172 |
150 |
2.13e-10 |
| probe |
100.0 |
25 bp |
0 |
0 |
28 |
52 |
1.84e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
