JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CQ947247
Database:	nrnl1	Go to original record
Description:	Sequence 48 from Patent WO2004099422. Sequence 24 from Patent WO2004111251. Sequence 26 from Patent WO2005075506. Sequence 49 from patent US 7232899. Adenovirus vector, packaging cell line, composition and method for production and use. ADENOVIRUS PARTICLES WITH ENHANCED INFECTIVITY OF DENDRITIC CELLS AND PARTICLES WITH DECREASED INFECTIVITY OF HEPATOCYTES. VECTORS FOR OCULAR TRANSDUCTION AND USE THEREFOR FOR GENETIC THERAPY. MODIFIED FIBER PROTEINS FOR EFFICIENT RECEPTOR BINDING. Fiber Shaft Modifications for Efficient Targeting. Fiber Shaft Modifications for Efficient Targeting.
Taxon:	unidentified
Original size:	11600 bp
Patents:	WO2004099422 (lens, espacenet) WO2004111251 (lens, espacenet) WO2005075506 (lens, espacenet) US7232899 (lens, espacenet) US2003157688 (lens, espacenet) JP2002534130 (lens, espacenet) JP2007525166 (lens, espacenet) JP2003531609 (lens, espacenet) JP2007531507 (lens, espacenet) JP2009034109 (lens, espacenet)

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Method:	QL-ELE-00-002	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:	nptII

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	6625	6839	+	215 bp

Amplicon:

215 bp

1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	3.06e-09
primerB	100.0	21 bp	0	0	215	195	3.06e-09

Match details