European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS409983
Database: nrnl1 Go to original record
Description: Sequence 13 from Patent EP1698699. Sequence 13 from patent US 6501009. Sequence 13 from patent US 6943281. Sequence 13 from patent US 7408096. Sequence 13 from patent US 8101826.
Taxon: unidentified
Original size: 4149 bp
Patents: EP1698699 (lens, espacenet) US6501009 (lens, espacenet) US6501009 (lens, espacenet) US6943281 (lens, espacenet) US2003115630 (lens, espacenet) US7408096 (lens, espacenet) US2005273882 (lens, espacenet) US8101826 (lens, espacenet)

View other 14 matches for this record

Method: QL-ELE-00-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target:T-nos

View other 15584 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3984 4067 + 84 bp
Amplicon: 84 bp 
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.