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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ871363
Database: nrnl1 Go to original record
Description: Sequence 15 from Patent WO2004078983. Sequence 1 from Patent WO2005095117. Sequence 1 from Patent WO2005098121. Use of hydroxypropylated high amylose content potato starches to achieve high kit numbers. Enhanced Amylose Production in Plants. Amylose starch product as sizing agents for textilesizing. Use of hydroxypropylated potato starch forobtaining high kit numbers. Enhanced Amylose Production in Plants. Amylose starch products as sizing agents for textile yarns.
Taxon: unidentified
Original size: 15294 bp
Patents: WO2004078983 (lens, espacenet) WO2005095117 (lens, espacenet) WO2005098121 (lens, espacenet) JP2007532339 (lens, espacenet) JP2006521099 (lens, espacenet) JP2007530809 (lens, espacenet)

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 8010 8174 + 165 bp
Amplicon: 165 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
51    cgactctagt cgagggccca tgggagcttg gattgaacaa gatggattgc
101   acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
151   gcacaacaga caatc

View other 133 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.04e-10
primerB 100.0 22 bp 0 0 165 144 6.90e-10
probe 100.0 25 bp 0 0 142 118 1.76e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.