JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CS646799
Database:	nrnl1	Go to original record
Description:	Sequence 54 from Patent WO2007061845. Sequence 90 from Patent WO2007061742. Sequence 54 from Patent WO2007127381. DELTA 15 DESATURASES SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST. DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 90 from patent US 7645604. Sequence 78 from patent US 7659120. Sequence 54 from patent US 7943823. Sequence 90 from patent US 8048653. Sequence 54 from patent US 8049062. Sequence 54 from patent US 8058517. Sequence 78 from patent US 8273957. Sequence 54 from Patent EP2405012.
Taxon:	unidentified
Original size:	9088 bp
Patents:	WO2007061845 (lens, espacenet) WO2007061742 (lens, espacenet) WO2007127381 (lens, espacenet) WO2007127381 (lens, espacenet) JP2007515951 (lens, espacenet) JP2009517019 (lens, espacenet) US7645604 (lens, espacenet) US7659120 (lens, espacenet) US7943823 (lens, espacenet) US8048653 (lens, espacenet) US8049062 (lens, espacenet) US8058517 (lens, espacenet) US8273957 (lens, espacenet) EP2405012 (lens, espacenet)

Method:	QL-ELE-00-009	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:	T-nos

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	4358	4475	+	118 bp

Amplicon:

118 bp

1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	24 bp	0	0	1	24	4.22e-11
primerB	100.0	24 bp	0	0	118	95	4.22e-11

Match details