Match details

Record ID: NRN_CS646845
Database: nrnl1 Go to original record
Description: Sequence 100 from Patent WO2007061845. Sequence 31 from Patent WO2008147935. Sequence 100 from patent US 8049062. Sequence 110 from patent US 8084074. Sequence 31 from patent US 8143473. Sequence 31 from patent US 8143476. Sequence 31 from patent US 8153859. Sequence 31 from Patent WO2009143401. Sequence 31 from Patent WO2009143398.
Taxon: unidentified
Original size: 15114 bp
Patents: WO2007061845 (lens, espacenet) WO2008147935 (lens, espacenet) US8049062 (lens, espacenet) US8084074 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3890 3968 - 79 bp

Amplicon: 79 bp
1     cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga 

View other 6569 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.