Match details
Record ID:
NRN_CS646845
Database:
nrnl1
Go to original record
Description:
Sequence 100 from Patent WO2007061845. Sequence 31 from Patent WO2008147935. Sequence 100 from patent US 8049062. Sequence 110 from patent US 8084074. Sequence 31 from patent US 8143473. Sequence 31 from patent US 8143476. Sequence 31 from patent US 8153859. Sequence 31 from Patent WO2009143401. Sequence 31 from Patent WO2009143398.
Taxon:
unidentified
Original size:
15114 bp
Patents:
WO2007061845 (lens , espacenet )
WO2008147935 (lens , espacenet )
US8049062 (lens , espacenet )
US8084074 (lens , espacenet )
US8143473 (lens , espacenet )
US8143476 (lens , espacenet )
US8153859 (lens , espacenet )
WO2009143401 (lens , espacenet )
WO2009143398 (lens , espacenet )
View other 14 matches for this record
Method:
QL-ELE-00-013
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target: T-nos
View other 15584 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
2187
2270
-
84 bp
1 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51 cgcaattata catttaatac gcgatagaaa acaa
View other 30799 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
25 bp
0
0
1
25
9.20e-12
primerB
100.0
25 bp
0
0
84
60
9.20e-12
probe
100.0
28 bp
0
0
28
55
2.28e-13
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.