Match details

Record ID: NRN_CS540096
Database: nrnl1 Go to original record
Description: Sequence 14 from Patent WO2007012078. Sequence 1 from Patent WO2007051725. Sequence 1 from Patent WO2007135188. METHIONINE PRODUCING RECOMBINANT MICROORGANISMS. Microorganism and process for the preparation of L-methionine. Method of producing methionine in Corynebacteria by over-expressing enzymes of the pentose phosphate pathway. Coryneform bacteria with formate-THF-synthetase and/or glycine cleavage activity. Sequence 31 from Patent WO2008101850. Sequence 28 from Patent WO2008101857. Sequence 20 from Patent WO2008152016. Sequence 1 from patent US 8148117. Sequence 32 from patent US 8163532. Sequence 32 from Patent WO2009144270.
Taxon: unidentified
Original size: 5477 bp
Patents: WO2007012078 (lens, espacenet) WO2007051725 (lens, espacenet) WO2007135188 (lens, espacenet) WO2007135188 (lens, espacenet) JP2009501550 (lens, espacenet) JP2009513119 (lens, espacenet) JP2010518827 (lens, espacenet) JP2010518837 (lens, espacenet) WO2008101850 (lens, espacenet) WO2008101857 (lens, espacenet) WO2008152016 (lens, espacenet) US8148117 (lens, espacenet) US8163532 (lens, espacenet) WO2009144270 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1924 2138 + 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.