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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ874879
Database: nrnl1 Go to original record
Description: Sequence 34 from Patent WO2004076617. Sequence 71 from Patent WO2004087902. Sequence 34 from patent US 7537920.
Taxon: unidentified
Original size: 17061 bp
Patents: WO2004076617 (lens, espacenet) WO2004087902 (lens, espacenet) US7537920 (lens, espacenet) US2006168687 (lens, espacenet)

View other 3 matches for this record

Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6815 6979 + 165 bp
Amplicon: 165 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
51    cgactctagt cgagggccca tgggagcttg gattgaacaa gatggattgc
101   acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
151   gcacaacaga caatc

View other 133 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.04e-10
primerB 100.0 22 bp 0 0 165 144 6.90e-10
probe 100.0 25 bp 0 0 142 118 1.76e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.