Match details

Record ID: NRN_AX481633
Database: nrnl1 Go to original record
Description: Sequence 23 from Patent WO02057465. Sequence 23 from Patent WO02057464. Sequence 41 from Patent WO03093482. Sequence 30 from Patent WO2004076617. Sequence 67 from Patent WO2004087902. Method for producing polyunsaturated fatty acids, novel biosynthesis genes and novel plant expression constructs. Sequence 30 from patent US 7537920. Sequence 23 from patent US 7615679. Sequence 41 from patent US 7893320. Sequence 23 from patent US 8088974.
Taxon: unidentified
Original size: 13002 bp
Patents: WO02057465 (lens, espacenet) WO02057464 (lens, espacenet) WO03093482 (lens, espacenet) WO2004076617 (lens, espacenet) WO2004087902 (lens, espacenet) JP2004530418 (lens, espacenet) US7537920 (lens, espacenet) US2006168687 (lens, espacenet) US7615679 (lens, espacenet) US2004053379 (lens, espacenet) US7893320 (lens, espacenet) US8088974 (lens, espacenet)

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Method: QL-ELE-00-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase promoter.
Target:P-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 10259 10352 - 94 bp

Amplicon: 94 bp
1     aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51    cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctga 

View other 3895 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.03e-12
primerB 100.0 24 bp 0 0 94 71 3.54e-11
probe 100.0 26 bp 0 0 28 53 3.03e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.