Match details
Record ID: |
NRN_EA306374 |
Database: |
nrnl1 |
Go to original record |
Description: |
Sequence 52 from patent US 7285703. Plant like starches and the method of making them in hosts. |
Taxon: |
unidentified |
Original size: |
2423 bp |
Patents: |
US7285703 (lens, espacenet)
US2006150281 (lens, espacenet)
JP2001519664 (lens, espacenet)
|
Method: |
QT-TAX-ZM-006 |
Go to GMOMETHODS record |
Description: | Quantitative PCR method for detection of maize starch synthase IIb gene |
Target: | maize starch synthase IIb |
View other 41 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
293 |
443 |
+ |
151 bp |
|
1 ctcccaatcc tttgacatct gctccgaagc aaagtcagag cgctgcaatg
51 caaaacggaa cgagtggggg cagcagcgcg agcaccgccg cgccggtgtc
101 cggacccaaa gctgatcatc catcagctcc tgtcaccaag agagaaatcg
151 a
View other 38 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
6.29e-10 |
primerB |
100.0 |
23 bp |
0 |
0 |
151 |
129 |
1.86e-10 |
probe |
100.0 |
25 bp |
0 |
0 |
28 |
52 |
1.60e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.