Match details

Record ID: NRN_CS476615
Database: nrnl1 Go to original record
Description: Sequence 14 from Patent WO2006136601. Sequence 84 from Patent WO2007031550. Sequence 110 from Patent WO2006067122. Sequence 53 from Patent WO2006120230. Sequence 127 from Patent WO2007141278. Sequence 49 from Patent WO2007141274. Sequence 141 from Patent WO2008028946. Sequence 110 from patent US 7244430. Human binding molecules having killing activity against staphylococci and uses thereof. Human binding molecules having killing activity against enterococci and uses thereof. Human binding molecules capable of neutralizing influenza virus H5N1 and uses thereof. Sequence 110 from patent US 7537764. Sequence 127 from patent US 7960518. Sequence 53 from patent US 8052974. Sequence 141 from patent US 8192927. Sequence 49 from patent US 8211431. Sequence 127 from patent US 8241631. Sequence 188 from Patent WO2010130636.
Taxon: unidentified
Original size: 10515 bp
Patents: WO2006136601 (lens, espacenet) WO2007031550 (lens, espacenet) WO2006067122 (lens, espacenet) WO2006120230 (lens, espacenet) WO2006120230 (lens, espacenet) WO2007141278 (lens, espacenet) WO2007141278 (lens, espacenet) WO2007141274 (lens, espacenet) WO2007141274 (lens, espacenet) WO2008028946 (lens, espacenet) US7244430 (lens, espacenet) US2007122801 (lens, espacenet) JP2009539360 (lens, espacenet) JP2009542194 (lens, espacenet) JP2010502207 (lens, espacenet) US7537764 (lens, espacenet) US2007042359 (lens, espacenet) US7960518 (lens, espacenet) US8052974 (lens, espacenet) US8192927 (lens, espacenet) US8211431 (lens, espacenet) US8241631 (lens, espacenet) WO2010130636 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 7538 7752 + 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.