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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_BD394368
Database: nrnl1 Go to original record
Description: A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences. A construct capable of release in closed circular form from alarger nucleotide sequence permitting site specific expressionand/or developmentally regulated expression of selected geneticsequences. Sequence 70 from patent US 7863430.
Taxon: unidentified
Original size: 2315 bp
Patents: JP2003528606 (lens, espacenet) US7863430 (lens, espacenet)

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Method: QL-ELE-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2092 2271 + 180 bp
Amplicon: 180 bp 
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tagcgcgcaa actaggataa

View other 29619 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.54e-09
primerB 100.0 20 bp 0 0 180 161 8.54e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.