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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX299821
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO0167855. Sequence 1 from Patent WO0204621. Sequence 1 from patent US 6730821. Sequence 1 from patent US 6815185. Sequence 1 from patent US 6942995. TRANSGENIC MICE CONTAINING TARGETED GENE DISRUPTIONS. TRANSGENIC MICE CONTAINING TRP GENE DISRUPTIONS. TRANSGENIC MICE CONTAINING TRP GENE DISRUPTIONS.
Taxon: unidentified
Original size: 4768 bp
Patents: WO0167855 (lens, espacenet) WO0204621 (lens, espacenet) US6730821 (lens, espacenet) US2004040046 (lens, espacenet) US6815185 (lens, espacenet) US2002086369 (lens, espacenet) US6942995 (lens, espacenet) US2002197624 (lens, espacenet) JP2004500102 (lens, espacenet) JP2003531573 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3433 3605 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.