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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX350578
Database: nrnl1 Go to original record
Description: Sequence 13 from Patent WO0183529. Sequence 13 from Patent WO2007044115. NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING VIRAL AND BACTERIAL DISEASES. IMPROVED CHIMERIC TOXIN PROTEINS AND CHIMERIC TOXIN RECEPTOR PROTEINS FOR TREATMENT AND PREVENTION OF ANTHRAX. JP 2010241817-A/8: NOVEL IMMUNOADHESINS FOR TREATING AND PREVENTING TOXICITY AND PATHOGEN-MEDIATED DISEASES. Sequence 13 from patent US 7951378.
Taxon: unidentified
Original size: 4465 bp
Patents: WO0183529 (lens, espacenet) WO2007044115 (lens, espacenet) JP2005522998 (lens, espacenet) JP2009502205 (lens, espacenet) JP2010241817 (lens, espacenet) US7951378 (lens, espacenet)

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Method: QL-ELE-00-008 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase promoter.
Target:P-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2531 2624 + 94 bp
Amplicon: 94 bp 
1     aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51    cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctga

View other 3895 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 26 bp 0 0 1 26 3.03e-12
primerB 100.0 24 bp 0 0 94 71 3.54e-11
probe 100.0 26 bp 0 0 28 53 3.03e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.