JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_FW567564
Database:	nrnl1	Go to original record
Description:	PRODUCTION OF OIL IN MICROORGANISMS. Sequence 8 from patent US 7883882. Sequence 8 from patent US 7935515. Sequence 8 from patent US 8187860. Sequence 8 from patent US 8222010. Sequence 8 from patent US 8268610. Sequence 25 from Patent EP2152849. JP 2012510275-A/3: PRODUCTION OF TAILORED OILS IN HETEROTROPHIC MICROORGANISMS. JP 2012513743-A/3: MANUFACTURING OF TAILORED OILS IN RECOMBINANT HETEROTROPHIC MICROORGANISMS. Sequence 25 from Patent EP2351845.
Taxon:	unidentified
Original size:	2615 bp
Patents:	JP2010528627 (lens, espacenet) US7883882 (lens, espacenet) US7935515 (lens, espacenet) US8187860 (lens, espacenet) US8222010 (lens, espacenet) US8268610 (lens, espacenet) EP2152849 (lens, espacenet) JP2012510275 (lens, espacenet) JP2012513743 (lens, espacenet) EP2351845 (lens, espacenet)

Method:	QL-ELE-00-017	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:	CaMV P-35S

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	640	714	+	75 bp

Amplicon:

75 bp

1     aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
51    aatcccacta tccttcgcaa gaccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	23 bp	0	0	1	23	9.50e-11
primerB	100.0	20 bp	0	0	75	56	3.68e-09

Match details