JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_AX107926
Database:	nrnl1	Go to original record
Description:	Sequence 54 from Patent WO0125466. Sequence 13 from Patent WO0175135. Sequence 71 from Patent WO0175135. Sequence 18 from Patent WO0179518. PRODUCER CELL FOR THE PRODUCTION OF RETROVIRAL VECTORS. CODON OPTIMISATION FOR EXPRESSION IN RETROVIRUS PACKAGING CELLS. Retinoic acid receptor beta-2, its antagonists, and gene therapy vectors for the treatment of neurological disorders. Retinoic acid receptor beta-2, its antagonists, and gene therapy vectors for the treatment of neurological disorders.
Taxon:	unidentified
Original size:	10114 bp
Patents:	WO0125466 (lens, espacenet) WO0175135 (lens, espacenet) WO0175135 (lens, espacenet) WO0179518 (lens, espacenet) JP2003511039 (lens, espacenet) JP2004508808 (lens, espacenet) JP2003533184 (lens, espacenet) JP2003533184 (lens, espacenet)

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Method:	QL-ELE-00-002	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:	nptII

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	7418	7632	+	215 bp

Amplicon:

215 bp

1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	3.06e-09
primerB	100.0	21 bp	0	0	215	195	3.06e-09

Match details