Match details
Record ID:
NRN_FW567564
Database:
nrnl1
Go to original record
Description:
PRODUCTION OF OIL IN MICROORGANISMS. Sequence 8 from patent US 7883882. Sequence 8 from patent US 7935515. Sequence 8 from patent US 8187860. Sequence 8 from patent US 8222010. Sequence 8 from patent US 8268610. Sequence 25 from Patent EP2152849. JP 2012510275-A/3: PRODUCTION OF TAILORED OILS IN HETEROTROPHIC MICROORGANISMS. JP 2012513743-A/3: MANUFACTURING OF TAILORED OILS IN RECOMBINANT HETEROTROPHIC MICROORGANISMS. Sequence 25 from Patent EP2351845.
Taxon:
unidentified
Original size:
2615 bp
Patents:
JP2010528627 (lens , espacenet )
US7883882 (lens , espacenet )
US7935515 (lens , espacenet )
US8187860 (lens , espacenet )
US8222010 (lens , espacenet )
US8268610 (lens , espacenet )
EP2152849 (lens , espacenet )
JP2012510275 (lens , espacenet )
JP2012513743 (lens , espacenet )
EP2351845 (lens , espacenet )
View other 9 matches for this record
Method:
QL-ELE-00-012
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target: CaMV P-35S
View other 15625 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
232
313
+
82 bp
1 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 26489 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
18 bp
0
0
1
18
4.54e-08
primerB
100.0
22 bp
0
0
82
61
3.55e-10
probe
100.0
22 bp
0
0
21
42
3.55e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.