Match details

Record ID: NRN_FW567564
Database: nrnl1 Go to original record
Description: PRODUCTION OF OIL IN MICROORGANISMS. Sequence 8 from patent US 7883882. Sequence 8 from patent US 7935515. Sequence 8 from patent US 8187860. Sequence 8 from patent US 8222010. Sequence 8 from patent US 8268610. Sequence 25 from Patent EP2152849. JP 2012510275-A/3: PRODUCTION OF TAILORED OILS IN HETEROTROPHIC MICROORGANISMS. JP 2012513743-A/3: MANUFACTURING OF TAILORED OILS IN RECOMBINANT HETEROTROPHIC MICROORGANISMS. Sequence 25 from Patent EP2351845.
Taxon: unidentified
Original size: 2615 bp
Patents: JP2010528627 (lens, espacenet) US7883882 (lens, espacenet) US7935515 (lens, espacenet) US8187860 (lens, espacenet) US8222010 (lens, espacenet) US8268610 (lens, espacenet) EP2152849 (lens, espacenet) JP2012510275 (lens, espacenet) JP2012513743 (lens, espacenet) EP2351845 (lens, espacenet)

View other 9 matches for this record


Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

View other 15625 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 232 313 + 82 bp

Amplicon: 82 bp
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt 

View other 26489 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.