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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX359933
Database: nrnl1 Go to original record
Description: Sequence 7 from Patent WO0202783. Sequence 7 from Patent WO0202738. Sequence 7 from patent US 6852510. Sequence 7 from patent US 7332333. Sequence 7 from patent US 7378273. Production of Host Cells Containing Multiple Integrating Vectors by Serial Transduction. Expression Vectors. Host Cells Containing Multiple Integrating Vectors. Expression Vectors. Host Cells Containing Multiple Integrating Vectors. Sequence 7 from Patent EP1572912. JP 2012115272-A/7: Host Cells Containing Multiple Integrating Vectors.
Taxon: unidentified
Original size: 9183 bp
Patents: WO0202783 (lens, espacenet) WO0202738 (lens, espacenet) US6852510 (lens, espacenet) US2003092882 (lens, espacenet) US7332333 (lens, espacenet) US2005100952 (lens, espacenet) US7378273 (lens, espacenet) US2005060762 (lens, espacenet) JP2007525975 (lens, espacenet) JP2004524807 (lens, espacenet) JP2004524805 (lens, espacenet) EP1572912 (lens, espacenet) JP2012115272 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1807 1979 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.