Match details
| Record ID: |
NRN_BD014473 |
| Database: |
nrnl1 |
Go to original record |
| Description: |
Transgenic corn and method of detecting recombinant gene from foods containing the same. |
| Taxon: |
unidentified |
| Original size: |
1416 bp |
| Patents: |
JP2001136983 (lens, espacenet)
|
View other 6 matches for this record
| Method: |
QT-CON-00-004 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Quantitative PCR method for detection of the junction between the intron 1 from the maize hsp70 gene and a synthetic cry1A(b) gene. |
| Target: | IVS-HSP-cryIAb |
View other 13 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
1000 |
1112 |
+ |
113 bp |
|
1 gatgccttct ccctagtgtt gaccagtgtt actcacatag tctttgctca
51 tttcattgta atgcagatac caagcggcca tggacaacaa cccaaacatc
101 aacgagtgca tcc
View other 13 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
4.98e-10 |
| primerB |
100.0 |
21 bp |
0 |
0 |
113 |
93 |
1.68e-09 |
| probe |
100.0 |
26 bp |
0 |
0 |
65 |
90 |
3.47e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
